Review



hydroxy guanine 8 ohdg  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Novus Biologicals hydroxy guanine 8 ohdg
    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of <t>8-OHdG</t> ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    Hydroxy Guanine 8 Ohdg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydroxy guanine 8 ohdg/product/Novus Biologicals
    Average 94 stars, based on 23 article reviews
    hydroxy guanine 8 ohdg - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin"

    Article Title: Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin

    Journal: The EMBO Journal

    doi: 10.1038/s44318-024-00039-y

    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of 8-OHdG ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    Figure Legend Snippet: ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of 8-OHdG ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .

    Techniques Used: Immunostaining, Expressing, Control, Microscopy, Software, Quantitative Proteomics

    Table showing details of all antibodies used for the manuscript.
    Figure Legend Snippet: Table showing details of all antibodies used for the manuscript.

    Techniques Used:



    Similar Products

    hydroxy guanine 8 ohdg  (Novus Biologicals)
    94
    Novus Biologicals hydroxy guanine 8 ohdg
    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of <t>8-OHdG</t> ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    Hydroxy Guanine 8 Ohdg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydroxy guanine 8 ohdg/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    hydroxy guanine 8 ohdg - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    8 ohdg  (Rockland Immunochemicals)
    93
    Rockland Immunochemicals 8 ohdg
    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of <t>8-OHdG</t> ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    8 Ohdg, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 ohdg/product/Rockland Immunochemicals
    Average 93 stars, based on 1 article reviews
    8 ohdg - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    goat polyclonal antibody against 8-hydroxy-deoxy-guanine (8-ohdg) mbs536217  (MyBiosource Biotechnology)
    90
    MyBiosource Biotechnology goat polyclonal antibody against 8-hydroxy-deoxy-guanine (8-ohdg) mbs536217
    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of <t>8-OHdG</t> ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    Goat Polyclonal Antibody Against 8 Hydroxy Deoxy Guanine (8 Ohdg) Mbs536217, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody against 8-hydroxy-deoxy-guanine (8-ohdg) mbs536217/product/MyBiosource Biotechnology
    Average 90 stars, based on 1 article reviews
    goat polyclonal antibody against 8-hydroxy-deoxy-guanine (8-ohdg) mbs536217 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    8 hydroxy guanine  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology 8 hydroxy guanine
    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of <t>8-OHdG</t> ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .
    8 Hydroxy Guanine, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 hydroxy guanine/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
    8 hydroxy guanine - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of 8-OHdG ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .

    Journal: The EMBO Journal

    Article Title: Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin

    doi: 10.1038/s44318-024-00039-y

    Figure Lengend Snippet: ( A ) ROS-associated pathways upregulated in GSEA analysis of epidermal compartment in E18.5 cKO skin compared to controls ( A ). ( B ) IF immunostaining of cKO skin showing the expression of 8-OHdG ( G ) in E16.5 KO and control skin ( N = 3). ( C ) Quantification of temporal change in the expression of 8-OHdG expression in cKO and control skin ( N ≥ 3). ( D ) qPCR showing increased transcription of genes associated with ROS generation in the cKO epidermis compared to WT ( N ≥ 3). ( E ) qPCR showing increased transcription of genes associated with ROS scavenging in the cKO epidermis compared to WT ( N ≥ 3). ( F ) IF showing increased expression of Catalase and Glutathione in cKO skin compared to WT ( N = 3). ( G ) cKO skin showing reduced expression of 8-OHdG and HIF1α after treatment with NAC compared to controls ( N = 4). ( H ) cKO skin showing reduced expression of GLUT1 and LDHa quantified in ( J ) in NAC, treated cKO skin compared to controls ( N = 3). ( I ) Quantification for ( G ) ( N = 4). ( J ) Quantification for ( H ) ( N = 3). ( K ) Schematic showing ROS-mediated regulation of HIF1α in cKO skin. Data Information: All the microscope images ( B , F , G , H ) were quantified ( C , I , J ) in ImageJ software. The number of biological replicates ( N ) for each panel is given in the figure legend. Scale bars: 50 μm. Student’s t-test was performed for statistical analysis. * p ≤ 0.05, * *p ≤ 0.01, **** p ≤ 0.0001, ns = not significant (student’s t test). All graphs are presented as mean ± SEM. WT experimental values are normalized to 1 for relative quantification. .

    Article Snippet: 12. , 8 hydroxy guanine (8-OHdG) , Novus Biologicals , NB100-1508 , 1:200.

    Techniques: Immunostaining, Expressing, Control, Microscopy, Software, Quantitative Proteomics

    Table showing details of all antibodies used for the manuscript.

    Journal: The EMBO Journal

    Article Title: Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin

    doi: 10.1038/s44318-024-00039-y

    Figure Lengend Snippet: Table showing details of all antibodies used for the manuscript.

    Article Snippet: 12. , 8 hydroxy guanine (8-OHdG) , Novus Biologicals , NB100-1508 , 1:200.

    Techniques: